Description: Rabbit polyclonal antibody to NASP Immunogen: Recombinant full length protein of human NASP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: NASP Alternative Names: Nuclear autoantigenic sperm protein; NASPEntrez Gene (Human): 4678Entrez Gene (Mouse) : 50927Entrez Gene (Rat) : 298441SwissProt (Human): P49321SwissProt (Mouse) : Q99MD9SwissProt (Rat) : Q66HD3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NASP expression in SW620 (A), HL60 (B) whole cell lysates.
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  Immunohistochemical analysis of NASP staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.