Description: Rabbit polyclonal antibody to MYL2 Immunogen: Recombinant full length protein of human MYL2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) Gene Symbol: MYL2 Alternative Names: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform; MLC-2; MLC-2vEntrez Gene (Human): 4633Entrez Gene (Mouse) : 17906Entrez Gene (Rat) : 363925SwissProt (Human): P10916SwissProt (Mouse) : P51667SwissProt (Rat) : P08733Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MYL2 expression in MCF7 (A), mouse skeletal muscle (B) whole cell lysates.
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Immunofluorescent analysis of MYL2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.