Description: Rabbit polyclonal antibody to GDF8 Immunogen: Recombinant full length protein of human GDF8 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MSTN Alternative Names: GDF8; Growth/differentiation factor 8; GDF-8; MyostatinEntrez Gene (Human): 2660Entrez Gene (Mouse) : 17700Entrez Gene (Rat) : 29152SwissProt (Human): O14793SwissProt (Mouse) : O08689SwissProt (Rat) : O35312Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GDF8 expression in U937 (A), BT474 (B), mouse heart (C), mouse liver (D), rat skeletal muscle (E) whole cell lysates.
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  Immunohistochemical analysis of GDF8 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.