Description: Rabbit polyclonal antibody to NRAGE Immunogen: Recombinant full length protein of human NRAGE Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MAGED1 Alternative Names: NRAGE; Melanoma-associated antigen D1; MAGE tumor antigen CCF; MAGE-D1 antigen; Neurotrophin receptor-interacting MAGE homologEntrez Gene (Human): 9500Entrez Gene (Mouse) : 94275Entrez Gene (Rat) : 84469SwissProt (Human): Q9Y5V3SwissProt (Mouse) : Q9QYH6SwissProt (Rat) : Q9ES73Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NRAGE expression in A549 (A) whole cell lysates.
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Immunohistochemical analysis of NRAGE staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.