Description: Rabbit polyclonal antibody to LDHB Immunogen: Recombinant full length protein of human LDHB Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: LDHB Alternative Names: L-lactate dehydrogenase B chain; LDH-B; LDH heart subunit; LDH-H; Renal carcinoma antigen NY-REN-46Entrez Gene (Human): 3945Entrez Gene (Mouse) : 16832Entrez Gene (Rat) : 24534SwissProt (Human): P07195SwissProt (Mouse) : P16125SwissProt (Rat) : P42123Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of LDHB expression in A431 (A), Jurkat (B), A549 (C), mouse kidney (D), mouse lung (E), rat brain (F) whole cell lysates.
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Immunohistochemical analysis of LDHB staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.