Description: Rabbit polyclonal antibody to IKK epsilon Immunogen: Recombinant full length protein of human IKK epsilon Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: IKBKE Alternative Names: IKKE; IKKI; KIAA0151; Inhibitor of nuclear factor kappa-B kinase subunit epsilon; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; Inducible I kappa-B kinase; IKK-iEntrez Gene (Human): 9641Entrez Gene (Mouse) : 56489SwissProt (Human): Q14164SwissProt (Mouse) : Q9R0T8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of IKK epsilon expression in SW480 (A), MCF7 (B), mouse liver (C) whole cell lysates.
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  Immunohistochemical analysis of IKK epsilon staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.