Description: Rabbit polyclonal antibody to IGFBP5 Immunogen: Recombinant full length protein of human IGFBP5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: IGFBP5 Alternative Names: IBP5; Insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5Entrez Gene (Human): 3488Entrez Gene (Mouse) : 16011Entrez Gene (Rat) : 25285SwissProt (Human): P24593SwissProt (Mouse) : Q07079SwissProt (Rat) : P24594Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of IGFBP5 expression in PC3 (A), BT474 (B), mouse kidney (C), mouse lung (D) whole cell lysates.
-
Immunohistochemical analysis of IGFBP5 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.