Description: Rabbit polyclonal antibody to IDH1 Immunogen: Recombinant full length protein of human IDH1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: IDH1 Alternative Names: PICD; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; Cytosolic NADP-isocitrate dehydrogenase; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylaseEntrez Gene (Human): 3417Entrez Gene (Mouse) : 15926Entrez Gene (Rat) : 24479SwissProt (Human): O75874SwissProt (Mouse) : O88844SwissProt (Rat) : P41562Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IDH1 expression in A549 (A), Raji (B), mouse kidney (C), mouse liver (D) whole cell lysates.
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Immunofluorescent analysis of IDH1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.