Description: Rabbit polyclonal antibody to OMI Immunogen: Recombinant full length protein of human OMI Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: HTRA2 Alternative Names: OMI; PRSS25; Serine protease HTRA2 mitochondrial; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMIEntrez Gene (Human): 27429Entrez Gene (Mouse) : 64704SwissProt (Human): O43464SwissProt (Mouse) : Q9JIY5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of OMI expression in HT29 (A), MCF7 (B), mouse heart (C), mouse kidney (D) whole cell lysates.
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Immunohistochemical analysis of OMI staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.