Description: Rabbit polyclonal antibody to HCN1 Immunogen: KLH-conjugated synthetic peptide of human HCN1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: HCN1 Alternative Names: BCNG1; Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1; Brain cyclic nucleotide-gated channel 1; BCNG-1Entrez Gene (Human): 348980Entrez Gene (Mouse) : 15165SwissProt (Human): O60741SwissProt (Mouse) : O88704Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HCN1 expression in MCF7 (A), SHSY5Y (B), mouse brain (C), mouse lung (D) whole cell lysates.
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Immunohistochemical analysis of HCN1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.