Description: Rabbit polyclonal antibody to HAPLN1 Immunogen: Recombinant full length protein of human HAPLN1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HAPLN1 Alternative Names: CRTL1; Hyaluronan and proteoglycan link protein 1; Cartilage-linking protein 1; Cartilage-link protein; Proteoglycan link proteinEntrez Gene (Human): 1404Entrez Gene (Rat) : 29331SwissProt (Human): P10915SwissProt (Rat) : P03994Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HAPLN1 expression in BT474 (A), MCF7 (B), Hela (C), HepG2 (D) whole cell lysates.
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Immunohistochemical analysis of HAPLN1 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.