Description: Rabbit polyclonal antibody to GTF2I Immunogen: KLH-conjugated synthetic peptide of human GTF2I Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GTF2I Alternative Names: BAP135; WBSCR6; General transcription factor II-I; GTFII-I; TFII-I; Bruton tyrosine kinase-associated protein 135; BAP-135; BTK-associated protein 135; SRF-Phox1-interacting protein; SPIN; Williams-Beuren syndrome chromosomal region 6 proteinEntrez Gene (Human): 2969Entrez Gene (Mouse) : 14886Entrez Gene (Rat) : 353256SwissProt (Human): P78347SwissProt (Mouse) : Q9ESZ8SwissProt (Rat) : Q5U2Y1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GTF2I expression in MCF7 (A), BT474 (B), Hela (C) whole cell lysates.
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Immunohistochemical analysis of GTF2I staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.