Description: Rabbit polyclonal antibody to GADD45 alpha Immunogen: Recombinant full length protein of human GADD45 alpha Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: GADD45A Alternative Names: DDIT1; GADD45; Growth arrest and DNA damage-inducible protein GADD45 alpha; DNA damage-inducible transcript 1 protein; DDIT-1Entrez Gene (Human): 1647Entrez Gene (Mouse) : 13197SwissProt (Human): P24522SwissProt (Mouse) : P48316SwissProt (Rat) : P48317Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of GADD45 alpha expression in mouse liver (A), mouse kidney (B) whole cell lysates.
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  Immunohistochemical analysis of GADD45 alpha staining in mouse testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.