Description: Rabbit polyclonal antibody to Ficolin 1 Immunogen: Recombinant full length protein of human Ficolin 1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: FCN1 Alternative Names: FCNM; Ficolin-1; Collagen/fibrinogen domain-containing protein 1; Ficolin-A; Ficolin-alpha; M-ficolinEntrez Gene (Human): 2219Entrez Gene (Mouse) : 14133SwissProt (Human): O00602SwissProt (Mouse) : O70165SwissProt (Rat) : Q9WTS8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ficolin 1 expression in HepG2 (A), mouse liver (B), mouse heart (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of Ficolin 1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.