Description: Rabbit polyclonal antibody to EZH2 Immunogen: Recombinant full length protein of human EZH2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/50 - 1/100) Gene Symbol: EZH2 Alternative Names: KMT6; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6Entrez Gene (Human): 2146Entrez Gene (Mouse) : 14056SwissProt (Human): Q15910SwissProt (Mouse) : Q61188Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of EZH2 expression in HL60 (A), SW620 (B), MCF7 (C), mouse ovary (D) whole cell lysates.
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  Immunohistochemical analysis of EZH2 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunoprecipitation of EZH2 from 0.5mg Hela whole cell extract lysate, using Anti-EZH2 Antibody.