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Anti-EZH2 Antibody CQA1951
  • Western blot analysis of EZH2 expression in HL60 (A), SW620 (B), MCF7 (C), mouse ovary (D) whole cell lysates.
  • Immunohistochemical analysis of EZH2 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of EZH2 from 0.5mg Hela whole cell extract lysate, using Anti-EZH2 Antibody.
Product NameAnti-EZH2 Antibody
Cat No: CQA1951
Source: Rabbit
Reactivity: H, M, R
Applications: WB, IH, IP
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
Size
Price
200 μl
$350
100 μl
$220
50 μl
$150
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Description: Rabbit polyclonal antibody to EZH2
Immunogen: Recombinant full length protein of human EZH2
Purification: The antibody was purified by immunogen affinity chromatography.
Clonality: Polyclonal
Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100), IP (1/50 - 1/100)
Gene Symbol: EZH2
Alternative Names: KMT6; Histone-lysine N-methyltransferase EZH2; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6
Entrez Gene (Human): 2146
Entrez Gene (Mouse) : 14056
SwissProt (Human): Q15910
SwissProt (Mouse) : Q61188
Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
  • Western blot analysis of EZH2 expression in HL60 (A), SW620 (B), MCF7 (C), mouse ovary (D) whole cell lysates.
  • Immunohistochemical analysis of EZH2 staining in human thyroid cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunoprecipitation of EZH2 from 0.5mg Hela whole cell extract lysate, using Anti-EZH2 Antibody.
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