Description: Rabbit polyclonal antibody to SMAC Immunogen: Recombinant full length protein of human SMAC Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: DIABLO Alternative Names: SMAC; Diablo homolog mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; SmacEntrez Gene (Human): 56616Entrez Gene (Mouse) : 66593SwissProt (Human): Q9NR28SwissProt (Mouse) : Q9JIQ3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SMAC expression in SW620 (A), BT474 (B), mouse spleen (C) whole cell lysates.
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  Immunohistochemical analysis of SMAC staining in mouse testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.