Description: Rabbit polyclonal antibody to DHRS9 Immunogen: Recombinant full length protein of human DHRS9 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: DHRS9 Alternative Names: Dehydrogenase/reductase SDR family member 9; 3-alpha hydroxysteroid dehydrogenase; 3-alpha-HSD; NADP-dependent retinol dehydrogenase/reductase; RDH-E2; RDHL; Short-chain dehydrogenase/reductase retSDR8; Tracheobronchial epithelial cell-specific retinol dehydrogenase; RDH-TBEEntrez Gene (Human): 10170Entrez Gene (Mouse) : 241452Entrez Gene (Rat) : 170635SwissProt (Human): Q9BPW9SwissProt (Mouse) : Q58NB6SwissProt (Rat) : Q8VD48Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of DHRS9 expression in HT29 (A), THP1 (B), mouse pancreas (C), rat trachea (D) whole cell lysates.
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Immunofluorescent analysis of DHRS9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.