Description: Rabbit polyclonal antibody to Cyclin C Immunogen: Recombinant full length protein of human Cyclin C Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: CCNC Alternative Names: Cyclin-C; SRB11 homolog; hSRB11Entrez Gene (Human): 892Entrez Gene (Mouse) : 51813SwissProt (Human): P24863SwissProt (Mouse) : Q62447SwissProt (Rat) : P39947Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cyclin C expression in MCF7 (A), NIH3T3 (B), mouse brain (C), mouse lung (D), rat liver (E) whole cell lysates.
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Immunohistochemical analysis of Cyclin C staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.