Description: Rabbit polyclonal antibody to BAAT Immunogen: Recombinant full length protein of human BAAT Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: BAAT Alternative Names: Bile acid-CoA:amino acid N-acyltransferase; BACAT; BAT; Glycine N-choloyltransferase; Long-chain fatty-acyl-CoA hydrolaseEntrez Gene (Human): 570Entrez Gene (Mouse) : 12012Entrez Gene (Rat) : 29725SwissProt (Human): Q14032SwissProt (Mouse) : Q91X34SwissProt (Rat) : Q63276Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of BAAT expression in A549 (A), HepG2 (B), mouse liver (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of BAAT staining in human liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.