Description: Rabbit polyclonal antibody to ATP5O Immunogen: Recombinant full length protein of human ATP5O Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: ATP5O Alternative Names: ATPO; ATP synthase subunit O mitochondrial; Oligomycin sensitivity conferral protein; OSCPEntrez Gene (Human): 539Entrez Gene (Mouse) : 102641678;28080Entrez Gene (Rat) : 192241SwissProt (Human): P48047SwissProt (Mouse) : Q9DB20SwissProt (Rat) : Q06647Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ATP5O expression in A549 (A), SKOV3 (B), mouse liver (C), mouse lung (D), rat heart (E) whole cell lysates.
-
Immunohistochemical analysis of ATP5O staining in rat stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Immunofluorescent analysis of ATP5O staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).