Description: Rabbit polyclonal antibody to Annexin A11 Immunogen: Recombinant full length protein of human Annexin A11 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: ANXA11 Alternative Names: ANX11; Annexin A11; 56 kDa autoantigen; Annexin XI; Annexin-11; Calcyclin-associated annexin 50; CAP-50Entrez Gene (Human): 311Entrez Gene (Mouse) : 11744SwissProt (Human): P50995SwissProt (Mouse) : P97384Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Annexin A11 expression in Jurkat (A), SKOV3 (B), A549 (C), mouse kidney (D), rat brain (E) whole cell lysates.
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Immunofluorescent analysis of Annexin A11 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.