Description: Rabbit polyclonal antibody to AMBRA1 Immunogen: Recombinant full length protein of human AMBRA1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: AMBRA1 Alternative Names: KIAA1736; Activating molecule in BECN1-regulated autophagy protein 1Entrez Gene (Human): 55626Entrez Gene (Mouse) : 228361SwissProt (Human): Q9C0C7SwissProt (Mouse) : A2AH22Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of AMBRA1 expression in MCF7 (A), A431 (B) whole cell lysates.
-
Immunohistochemical analysis of AMBRA1 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.