Description: Rabbit polyclonal antibody to METTL3 Immunogen: Recombinant full length protein of human METTL3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/20 - 1/50) Gene Symbol: METTL3 Alternative Names: MTA70; N6-adenosine-methyltransferase 70 kDa subunit; MT-A70; Methyltransferase-like protein 3Entrez Gene (Human): 56339Entrez Gene (Mouse) : 56335SwissProt (Human): Q86U44SwissProt (Mouse) : Q8C3P7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of METTL3 expression in HepG2 (A), mouse spleen (B) whole cell lysates.
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  Immunohistochemical analysis of METTL3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of METTL3 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.