Description: Rabbit polyclonal antibody to MT-ND5 Immunogen: KLH-conjugated synthetic peptide of mouse MT-ND5 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MT-ND5 Alternative Names: MTND5; NADH5; ND5; NADH-ubiquinone oxidoreductase chain 5; NADH dehydrogenase subunit 5Entrez Gene (Human): 4540Entrez Gene (Mouse) : 17721SwissProt (Human): P03915SwissProt (Mouse) : P03921SwissProt (Rat) : P11661Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MT-ND5 expression in mouse brain (A) whole cell lysates.
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Immunohistochemical analysis of MT-ND5 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.