Description: Rabbit polyclonal antibody to PIP4K2 beta Immunogen: Recombinant full length protein of human PIP4K2 beta Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) Gene Symbol: PIP4K2B Alternative Names: PIP5K2B; Phosphatidylinositol 5-phosphate 4-kinase type-2 beta; 1-phosphatidylinositol 5-phosphate 4-kinase 2-beta; Diphosphoinositide kinase 2-beta; Phosphatidylinositol 5-phosphate 4-kinase type II beta; PI(5)P 4-kinase type II beta; PIP4KII-beta; PtdIns(5)P-4-kinase isoform 2-betaEntrez Gene (Human): 8396Entrez Gene (Mouse) : 108083Entrez Gene (Rat) : 89812SwissProt (Human): P78356SwissProt (Mouse) : Q80XI4SwissProt (Rat) : O88377Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PIP4K2 beta expression in Hela (A), A549 (B), mouse spleen (C) whole cell lysates.
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  Immunohistochemical analysis of PIP4K2 beta staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PIP4K2 beta staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.