Description: Rabbit polyclonal antibody to SNAP-alpha Immunogen: Recombinant full length protein of human SNAP-alpha Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NAPA Alternative Names: SNAPA; Alpha-soluble NSF attachment protein; SNAP-alpha; N-ethylmaleimide-sensitive factor attachment protein alphaEntrez Gene (Human): 8775Entrez Gene (Mouse) : 108124Entrez Gene (Rat) : 140673SwissProt (Human): P54920SwissProt (Mouse) : Q9DB05SwissProt (Rat) : P54921Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SNAP-alpha expression in Hela (A), Jurkat (B), mouse brain (C), mouse lung (D), rat liver (E) whole cell lysates.
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Immunohistochemical analysis of SNAP-alpha staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.