Description: Rabbit polyclonal antibody to Caprin1 Immunogen: Recombinant full length protein of human Caprin1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: CAPRIN1 Alternative Names: GPIAP1; GPIP137; M11S1; RNG105; Caprin-1; Cell cycle-associated protein 1; Cytoplasmic activation- and proliferation-associated protein 1; GPI-anchored membrane protein 1; GPI-anchored protein p137; GPI-p137; p137GPI; Membrane component chromosome 11 surface marker 1; RNA granule protein 105Entrez Gene (Human): 4076Entrez Gene (Mouse) : 53872Entrez Gene (Rat) : 362173SwissProt (Human): Q14444SwissProt (Mouse) : Q60865SwissProt (Rat) : Q5M9G3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Caprin1 expression in Hela (A), Jurkat (B) whole cell lysates.
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  Immunofluorescent analysis of Caprin1 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).