Description: Rabbit polyclonal antibody to eIF2B1 Immunogen: Recombinant full length protein of human eIF2B1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: EIF2B1 Alternative Names: EIF2BA; Translation initiation factor eIF-2B subunit alpha; eIF-2B GDP-GTP exchange factor subunit alphaEntrez Gene (Human): 1967Entrez Gene (Mouse) : 209354SwissProt (Human): Q14232SwissProt (Mouse) : Q99LC8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of eIF2B1 expression in SHSY5Y (A), MCF7 (B), mouse liver (C), mouse brain (D) whole cell lysates.
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Immunofluorescent analysis of eIF2B1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).