Description: Rabbit polyclonal antibody to AspRS Immunogen: Recombinant full length protein of human AspRS Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: DARS2 Alternative Names: Aspartate--tRNA ligase mitochondrial; Aspartyl-tRNA synthetase; AspRSEntrez Gene (Human): 55157SwissProt (Human): Q6PI48Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AspRS expression in NCIH460 (A), U251 (B), MCF7 (C), BT474 (D), mouse liver (E), mouse heart (F) whole cell lysates.
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Immunohistochemical analysis of AspRS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.