Description: Rabbit polyclonal antibody to Cbl-3 Immunogen: Recombinant full length protein of human Cbl-3 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) Gene Symbol: CBLC Alternative Names: CBL3; RNF57; E3 ubiquitin-protein ligase CBL-C; RING finger protein 57; SH3-binding protein CBL-3; SH3-binding protein CBL-C; Signal transduction protein CBL-CEntrez Gene (Human): 23624SwissProt (Human): Q9ULV8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Cbl-3 expression in HEK293T (A), Raji (B), mouse kidney (C) whole cell lysates.
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Immunofluorescent analysis of Cbl-3 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.