Description: Rabbit polyclonal antibody to p62 Immunogen: Recombinant full length protein of human p62 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SQSTM1 Alternative Names: ORCA; OSIL; Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62Entrez Gene (Human): 8878Entrez Gene (Mouse) : 18412Entrez Gene (Rat) : 113894SwissProt (Human): Q13501SwissProt (Mouse) : Q64337SwissProt (Rat) : O08623Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of p62 expression in Hela (A), Jurkat (B), mouse liver (C), mouse kidney (D) whole cell lysates.
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  Immunohistochemical analysis of p62 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.