Description: Rabbit polyclonal antibody to RCN2 Immunogen: Recombinant full length protein of human RCN2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RCN2 Alternative Names: ERC55; Reticulocalbin-2; Calcium-binding protein ERC-55; E6-binding protein; E6BPEntrez Gene (Human): 5955SwissProt (Human): Q14257Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RCN2 expression in Hela (A), MCF7 (B) whole cell lysates.
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Immunohistochemical analysis of RCN2 staining in rat heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.