Description: Rabbit polyclonal antibody to OSGEP Immunogen: Recombinant full length protein of human OSGEP Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: OSGEP Alternative Names: GCPL1; Probable tRNA N6-adenosine threonylcarbamoyltransferase; N6-L-threonylcarbamoyladenine synthase; t(6)A synthase; O-sialoglycoprotein endopeptidase; hOSGEP; t(6)A37 threonylcarbamoyladenosine biosynthesis protein OSGEP; tRNA threonylcarbamoyladenosine biosynthesis protein OSGEPEntrez Gene (Human): 55644Entrez Gene (Mouse) : 66246Entrez Gene (Rat) : 290028SwissProt (Human): Q9NPF4SwissProt (Mouse) : Q8BWU5SwissProt (Rat) : Q9WVS2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of OSGEP expression in Raji (A), Jurkat (B), mouse heart (C), mouse ovary (D), rat liver (E) whole cell lysates.
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Immunofluorescent analysis of OSGEP staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.