Description: Rabbit polyclonal antibody to AUH Immunogen: Recombinant full length protein of human AUH Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: AUH Alternative Names: Methylglutaconyl-CoA hydratase mitochondrial; AU-specific RNA-binding enoyl-CoA hydratase; AU-binding protein/enoyl-CoA hydrataseEntrez Gene (Human): 549Entrez Gene (Mouse) : 11992SwissProt (Human): Q13825SwissProt (Mouse) : Q9JLZ3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AUH expression in A549 (A), SW480 (B), mouse brain (C), mouse kidney (D), rat heart (E) whole cell lysates.
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Immunohistochemical analysis of AUH staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.