Description: Rabbit polyclonal antibody to Ciao 1 Immunogen: Recombinant full length protein of human Ciao 1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: CIAO1 Alternative Names: WDR39; Probable cytosolic iron-sulfur protein assembly protein CIAO1; WD repeat-containing protein 39Entrez Gene (Human): 9391Entrez Gene (Mouse) : 26371Entrez Gene (Rat) : 29231SwissProt (Human): O76071SwissProt (Mouse) : Q99KN2SwissProt (Rat) : Q5M7T1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ciao 1 expression in Jurkat (A), mouse kidney (B), rat heart (C) whole cell lysates.
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Immunohistochemical analysis of Ciao 1 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.