Description: Rabbit polyclonal antibody to YT521-B Immunogen: Recombinant full length protein of human YT521-B Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) Gene Symbol: YTHDC1 Alternative Names: KIAA1966; YT521; YTH domain-containing protein 1; Putative splicing factor YT521; YT521-BEntrez Gene (Human): 91746Entrez Gene (Mouse) : 231386Entrez Gene (Rat) : 170956SwissProt (Human): Q96MU7SwissProt (Mouse) : E9Q5K9SwissProt (Rat) : Q9QY02Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of YT521-B expression in MCF7 (A), Jurkat (B), mouse brain (C), rat brain (D) whole cell lysates.
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  Immunofluorescent analysis of YT521-B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.