Description: Rabbit polyclonal antibody to UBE2J2 Immunogen: Recombinant full length protein of human UBE2J2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/100) Gene Symbol: UBE2J2 Alternative Names: NCUBE2; Ubiquitin-conjugating enzyme E2 J2; Non-canonical ubiquitin-conjugating enzyme 2; NCUBE-2Entrez Gene (Human): 118424Entrez Gene (Mouse) : 140499SwissProt (Human): Q8N2K1SwissProt (Mouse) : Q6P073Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of UBE2J2 expression in Hela (A), mouse testis (B), mouse brain (C) whole cell lysates.
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  Immunohistochemical analysis of UBE2J2 staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of UBE2J2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.