Anti-HEMK2 Antibody 
Datasheet
MSDS
Description: Rabbit polyclonal antibody to HEMK2 Immunogen: Recombinant full length protein of human HEMK2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: N6AMT1 Alternative Names: C21orf127; HEMK2; HemK methyltransferase family member 2; M.HsaHemK2P; N(6)-adenine-specific DNA methyltransferase 1Entrez Gene (Human): 29104SwissProt (Human): Q9Y5N5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HEMK2 expression in SKOV3 (A), mouse heart (B), mouse brain (C) whole cell lysates. -
Immunofluorescent analysis of HEMK2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
The methyltransferase N6AMT1 participates in the cell cycle by regulating cyclin E levels
Cross-Reactivity of N6AMT1 Antibodies with Aurora Kinase A: An Example of Antibody-Specific Non-Specificity