Description: Rabbit polyclonal antibody to DPP8 Immunogen: Recombinant full length protein of human DPP8 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/100) Gene Symbol: DPP8 Alternative Names: DPRP1; Dipeptidyl peptidase 8; DP8; Dipeptidyl peptidase IV-related protein 1; DPRP-1; Dipeptidyl peptidase VIII; DPP VIII; Prolyl dipeptidase DPP8Entrez Gene (Human): 54878Entrez Gene (Mouse) : 74388SwissProt (Human): Q6V1X1SwissProt (Mouse) : Q80YA7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of DPP8 expression in MCF7 (A), Hela (B), mouse brain (C), rat lung (D) whole cell lysates.
-
Immunofluorescent analysis of DPP8 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.