Description: Rabbit polyclonal antibody to PC4 Immunogen: Recombinant full length protein of human PC4 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200), IP (1/10 - 1/50) Gene Symbol: SUB1 Alternative Names: PC4; RPO2TC1; Activated RNA polymerase II transcriptional coactivator p15; Positive cofactor 4; PC4; SUB1 homolog; p14Entrez Gene (Human): 10923Entrez Gene (Mouse) : 20024SwissProt (Human): P53999SwissProt (Mouse) : P11031Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PC4 expression in HL60 (A), Jurkat (B), mouse brain (C) whole cell lysates.
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  Immunofluorescent analysis of PC4 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.