Description: Rabbit polyclonal antibody to eIF3C Immunogen: Recombinant full length protein of human eIF3C Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: EIF3C Alternative Names: EIF3S8; Eukaryotic translation initiation factor 3 subunit C; eIF3c; Eukaryotic translation initiation factor 3 subunit 8; eIF3 p110Entrez Gene (Human): 8663Entrez Gene (Mouse) : 56347Entrez Gene (Rat) : 293484SwissProt (Human): Q99613SwissProt (Mouse) : Q8R1B4SwissProt (Rat) : B5DFC8Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of eIF3C expression in HepG2 (A), SW620 (B), mouse testis (C) whole cell lysates.
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  Immunohistochemical analysis of eIF3C staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of eIF3C staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.