Description: Rabbit polyclonal antibody to Transgelin-2 Immunogen: Recombinant full length protein of human Transgelin-2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: TAGLN2 Alternative Names: KIAA0120; Transgelin-2; Epididymis tissue protein Li 7e; SM22-alpha homologEntrez Gene (Human): 8407Entrez Gene (Mouse) : 21346Entrez Gene (Rat) : 304983SwissProt (Human): P37802SwissProt (Mouse) : Q9WVA4SwissProt (Rat) : Q5XFX0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Transgelin-2 expression in HEK293T (A), Hela (B), THP1 (C), MCF7 (D), SKOV3 (E), mouse lung (F) whole cell lysates.
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  Immunofluorescent analysis of Transgelin-2 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.