Description: Rabbit polyclonal antibody to DARS Immunogen: Recombinant full length protein of human DARS Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: DARS Alternative Names: Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 proteinEntrez Gene (Human): 1615Entrez Gene (Mouse) : 226414Entrez Gene (Rat) : 116483SwissProt (Human): P14868SwissProt (Mouse) : Q922B2SwissProt (Rat) : P15178Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DARS expression in MCF7 (A), SW480 (B), mouse brain (C), rat liver (D) whole cell lysates.
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  Immunohistochemical analysis of DARS staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.