Description: Rabbit polyclonal antibody to AMCase Immunogen: Recombinant full length protein of human AMCase Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: CHIA Alternative Names: Acidic mammalian chitinase; AMCase; Lung-specific protein TSA1902Entrez Gene (Human): 27159Entrez Gene (Mouse) : 81600Entrez Gene (Rat) : 113901SwissProt (Human): Q9BZP6SwissProt (Mouse) : Q91XA9SwissProt (Rat) : Q6RY07Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of AMCase expression in HepG2 (A), mouse stomach (B), rat lung (C) whole cell lysates.
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Immunohistochemical analysis of AMCase staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.