Description: Rabbit polyclonal antibody to SHARP-2 Immunogen: Recombinant full length protein of human SHARP-2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: BHLHE40 Alternative Names: BHLHB2; DEC1; SHARP2; STRA13; Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2; bHLHb2; Differentially expressed in chondrocytes protein 1; DEC1; Enhancer-of-split and hairy-related protein 2; SHARP-2; Stimulated by retinoic acid gene 13 proteinEntrez Gene (Human): 8553Entrez Gene (Mouse) : 20893SwissProt (Human): O14503SwissProt (Mouse) : O35185Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SHARP-2 expression in HepG2 (A), mouse kidney (B) whole cell lysates.
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Immunohistochemical analysis of SHARP-2 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.