Description: Rabbit polyclonal antibody to HPPDase Immunogen: Recombinant full length protein of human HPPDase Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: HPD Alternative Names: PPD; 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; 4HPPD; HPD; HPPDaseEntrez Gene (Human): 3242Entrez Gene (Mouse) : 15445Entrez Gene (Rat) : 29531SwissProt (Human): P32754SwissProt (Mouse) : P49429SwissProt (Rat) : P32755Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of HPPDase expression in HepG2 (A), A549 (B), mouse kidney (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of HPPDase staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.