Description: Rabbit polyclonal antibody to DDAH2 Immunogen: Recombinant full length protein of human DDAH2 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: DDAH2 Alternative Names: DDAH; G6A; NG30; N(G),N(G)-dimethylarginine dimethylaminohydrolase 2; DDAH-2; Dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase proteinEntrez Gene (Human): 23564Entrez Gene (Mouse) : 51793Entrez Gene (Rat) : 294239SwissProt (Human): O95865SwissProt (Mouse) : Q99LD8SwissProt (Rat) : Q6MG60Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of DDAH2 expression in SW620 (A), MCF7 (B), mouse lung (C), mouse kidney (D), rat heart (E), rat brain (F) whole cell lysates.
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  Immunofluorescent analysis of DDAH2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.