Anti-PPOX Antibody-Primary Antibody-Cohesion Biosciences

Western blot analysis of PPOX expression in U2OS (A) whole cell lysates.
Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Name	Anti-PPOX Antibody
Cat No:	CQA1363
Source:	Rabbit
Reactivity:	H, M
Applications:	WB, IH
*Application Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
*Species Reactivity Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish

Size

Price

200 μl

$350

100 μl

$220

50 μl

$150

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Description: Rabbit polyclonal antibody to PPOX

Immunogen: Recombinant full length protein of human PPOX

Purification: The antibody was purified by immunogen affinity chromatography.

Clonality: Polyclonal

Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200)

Gene Symbol: PPOX

Alternative Names: Protoporphyrinogen oxidase; PPO

Entrez Gene (Human): 5498

Entrez Gene (Mouse) : 19044

SwissProt (Human): P50336

SwissProt (Mouse) : P51175

Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Western blot analysis of PPOX expression in U2OS (A) whole cell lysates.
Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study

Journal PLOS ONE

IF 3.24

Application WB

Reactivity Human

PMID 34793488

Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene

Journal Research Square

Application WB

Reactivity Human

PMID 36224252

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