Description: Rabbit polyclonal antibody to PPOX Immunogen: Recombinant full length protein of human PPOX Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: PPOX Alternative Names: Protoporphyrinogen oxidase; PPOEntrez Gene (Human): 5498Entrez Gene (Mouse) : 19044SwissProt (Human): P50336SwissProt (Mouse) : P51175Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PPOX expression in U2OS (A) whole cell lysates.
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Immunohistochemical analysis of PPOX staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study
Generation and characterization of human U-2 OS cell lines with the CRISPR/Cas9-edited protoporphyrinogen oxidase IX gene