Description: Rabbit polyclonal antibody to PCBD1 Immunogen: Recombinant full length protein of human PCBD1 Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/10 - 1/100) Gene Symbol: PCBD1 Alternative Names: DCOH; PCBD; Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCDEntrez Gene (Human): 5092Entrez Gene (Mouse) : 13180Entrez Gene (Rat) : 29700SwissProt (Human): P61457SwissProt (Mouse) : P61458SwissProt (Rat) : P61459Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PCBD1 expression in SW620 (A), mouse kidney (B), mouse pancreas (C), rat liver (D) whole cell lysates.
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  Immunofluorescent analysis of PCBD1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.