Description: Rabbit polyclonal antibody to Uev1A Immunogen: Recombinant full length protein of human Uev1A Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/10 - 1/100) Gene Symbol: UBE2V1 Alternative Names: CROC1; UBE2V; UEV1; Ubiquitin-conjugating enzyme E2 variant 1; UEV-1; CROC-1; TRAF6-regulated IKK activator 1 beta Uev1AEntrez Gene (Human): 387522; 7335Entrez Gene (Mouse) : 66589SwissProt (Human): Q13404SwissProt (Mouse) : Q9CZY3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Uev1A expression in HepG2 (A), Hela (B), mouse brain (C), rat liver (D), rat spleen (E) whole cell lysates.
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Immunofluorescent analysis of Uev1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.